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human oca cell lines es2  (ATCC)


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    ATCC human oca cell lines es2
    (A-B) <t>ES2,</t> BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.
    Human Oca Cell Lines Es2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+oca+cell+lines+es2/pmc05564823-112-0-12?v=ATCC
    Average 97 stars, based on 1227 article reviews
    human oca cell lines es2 - by Bioz Stars, 2026-07
    97/100 stars

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    1) Product Images from "Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer"

    Article Title: Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18442

    (A-B) ES2, BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.
    Figure Legend Snippet: (A-B) ES2, BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Techniques Used: MTT Assay, Cell Culture, Transduction, Expressing, Plasmid Preparation, Activity Assay

    (A-B) ES2 cells were pretreated with Liq (25 μM) for 48 h followed by treatment with varying doses of cytotoxic drugs paclitaxel or cisplatin for an additional 5 days. Cell viability was determined using MTT assay. (C) ES2 and SKOV3 cells were treated with vehicle, Liq or S-equol for 24 h and then used in transwell migration assays. Optical density was measured 16 h after migration. Photomicrographs of migrated cells in various treatments are shown. (D) Cell invasion potential of ES2 and SKOV3 cells treated with ERβ agonists was analyzed by using Matrigel invasion chamber assays. Photomicrographs of invaded cells in various treatments are shown. Data are represented as mean ± SE. ** p<0.01; *** p<0.001.
    Figure Legend Snippet: (A-B) ES2 cells were pretreated with Liq (25 μM) for 48 h followed by treatment with varying doses of cytotoxic drugs paclitaxel or cisplatin for an additional 5 days. Cell viability was determined using MTT assay. (C) ES2 and SKOV3 cells were treated with vehicle, Liq or S-equol for 24 h and then used in transwell migration assays. Optical density was measured 16 h after migration. Photomicrographs of migrated cells in various treatments are shown. (D) Cell invasion potential of ES2 and SKOV3 cells treated with ERβ agonists was analyzed by using Matrigel invasion chamber assays. Photomicrographs of invaded cells in various treatments are shown. Data are represented as mean ± SE. ** p<0.01; *** p<0.001.

    Techniques Used: MTT Assay, Migration

    Total RNA was isolated from the ES2 cells that were treated with either vehicle or Liq (100 μM) for 24 h and subjected to RNA sequencing. (A) Heat map of differentially expressed genes between vehicle and Liq is shown. (B) Differentially expressed genes were subjected to pathway analysis using IPA software, and the selected top canonical pathways are shown. Analysis of molecular and cellular functions of differentially expressed genes are shown. (C) Gene set enrichment analysis (GSEA) testing correlation of Liq-regulated genes with signatures of NF-κB signaling gene set and inflammatory response gene set. (D) ES2 and SKOV3 cells were treated with either vehicle or Liq or S-equol for 24 h, and the selective genes representing each pathway were validated by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.
    Figure Legend Snippet: Total RNA was isolated from the ES2 cells that were treated with either vehicle or Liq (100 μM) for 24 h and subjected to RNA sequencing. (A) Heat map of differentially expressed genes between vehicle and Liq is shown. (B) Differentially expressed genes were subjected to pathway analysis using IPA software, and the selected top canonical pathways are shown. Analysis of molecular and cellular functions of differentially expressed genes are shown. (C) Gene set enrichment analysis (GSEA) testing correlation of Liq-regulated genes with signatures of NF-κB signaling gene set and inflammatory response gene set. (D) ES2 and SKOV3 cells were treated with either vehicle or Liq or S-equol for 24 h, and the selective genes representing each pathway were validated by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Techniques Used: Isolation, RNA Sequencing, Software, Quantitative RT-PCR

    (A) ES2, SKOV3, and SKOV3-TR cells were transfected with NF-κB-luc reporter plasmid and grown for 24 h. Then, cells were treated with ERβ agonists and reporter activity was measured after 24 h. ES2 (B) and SKOV3 (C) cells were treated with Liq or S-equol (100 μM) for 24 h, and the expression status of NF-κB target genes was analyzed by using RT-qPCR. (D) ES2 cell lysates were used in pull-down assays using GST or ERβ-GST, and interaction of ERβ with p65 was analyzed by Western blotting. (E) ES2-ERβ-FLAG cell lysates were subjected to immunoprecipitation with IgG or FLAG antibodies and the interaction of ERβ with p65 was confirmed by Western blotting. (F) SKOV3 cells were transduced with empty vector or ERβ-FLAG expression vector and the expression of NF-κB target genes was analyzed by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.
    Figure Legend Snippet: (A) ES2, SKOV3, and SKOV3-TR cells were transfected with NF-κB-luc reporter plasmid and grown for 24 h. Then, cells were treated with ERβ agonists and reporter activity was measured after 24 h. ES2 (B) and SKOV3 (C) cells were treated with Liq or S-equol (100 μM) for 24 h, and the expression status of NF-κB target genes was analyzed by using RT-qPCR. (D) ES2 cell lysates were used in pull-down assays using GST or ERβ-GST, and interaction of ERβ with p65 was analyzed by Western blotting. (E) ES2-ERβ-FLAG cell lysates were subjected to immunoprecipitation with IgG or FLAG antibodies and the interaction of ERβ with p65 was confirmed by Western blotting. (F) SKOV3 cells were transduced with empty vector or ERβ-FLAG expression vector and the expression of NF-κB target genes was analyzed by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Techniques Used: Transfection, Plasmid Preparation, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunoprecipitation, Transduction



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    human oca cell lines es2  (ATCC)
    97
    ATCC human oca cell lines es2
    (A-B) <t>ES2,</t> BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.
    Human Oca Cell Lines Es2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+oca+cell+lines+es2/pmc05564823-112-0-12?v=ATCC
    Average 97 stars, based on 1 article reviews
    human oca cell lines es2 - by Bioz Stars, 2026-07
    97/100 stars
    		  Buy from Supplier

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    (A-B) ES2, BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Journal: Oncotarget

    Article Title: Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer

    doi: 10.18632/oncotarget.18442

    Figure Lengend Snippet: (A-B) ES2, BG-1, SKOV3, and SKOV3-TR cells were treated with vehicle or S-equol or Liq for 72 h, and the cell viability was measured by using a MTT assay. (C-D) ES2 and SKOV3 cells were treated with vehicle, S-equol, or Liq for 72 h and then cultured in growth medium for additional 7 days. The number of colonies for each group was counted. (E) ES2 cells were transduced with either empty or ERβ-FLAG expression vector and cell proliferation rates were measured using MTT assay. (F) ES2, SKOV3, and SKOV3-TR cells were treated with vehicle, S-equol, or Liq for 48 h, and the Caspase-3/7 activity was measured as described in Methods. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Article Snippet: Human OCa cell lines ES2, SKOV3, and BG-1 were obtained from the American Type Culture Collection (ATCC) and were maintained as per ATCC guidelines.

    Techniques: MTT Assay, Cell Culture, Transduction, Expressing, Plasmid Preparation, Activity Assay

    (A-B) ES2 cells were pretreated with Liq (25 μM) for 48 h followed by treatment with varying doses of cytotoxic drugs paclitaxel or cisplatin for an additional 5 days. Cell viability was determined using MTT assay. (C) ES2 and SKOV3 cells were treated with vehicle, Liq or S-equol for 24 h and then used in transwell migration assays. Optical density was measured 16 h after migration. Photomicrographs of migrated cells in various treatments are shown. (D) Cell invasion potential of ES2 and SKOV3 cells treated with ERβ agonists was analyzed by using Matrigel invasion chamber assays. Photomicrographs of invaded cells in various treatments are shown. Data are represented as mean ± SE. ** p<0.01; *** p<0.001.

    Journal: Oncotarget

    Article Title: Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer

    doi: 10.18632/oncotarget.18442

    Figure Lengend Snippet: (A-B) ES2 cells were pretreated with Liq (25 μM) for 48 h followed by treatment with varying doses of cytotoxic drugs paclitaxel or cisplatin for an additional 5 days. Cell viability was determined using MTT assay. (C) ES2 and SKOV3 cells were treated with vehicle, Liq or S-equol for 24 h and then used in transwell migration assays. Optical density was measured 16 h after migration. Photomicrographs of migrated cells in various treatments are shown. (D) Cell invasion potential of ES2 and SKOV3 cells treated with ERβ agonists was analyzed by using Matrigel invasion chamber assays. Photomicrographs of invaded cells in various treatments are shown. Data are represented as mean ± SE. ** p<0.01; *** p<0.001.

    Article Snippet: Human OCa cell lines ES2, SKOV3, and BG-1 were obtained from the American Type Culture Collection (ATCC) and were maintained as per ATCC guidelines.

    Techniques: MTT Assay, Migration

    Total RNA was isolated from the ES2 cells that were treated with either vehicle or Liq (100 μM) for 24 h and subjected to RNA sequencing. (A) Heat map of differentially expressed genes between vehicle and Liq is shown. (B) Differentially expressed genes were subjected to pathway analysis using IPA software, and the selected top canonical pathways are shown. Analysis of molecular and cellular functions of differentially expressed genes are shown. (C) Gene set enrichment analysis (GSEA) testing correlation of Liq-regulated genes with signatures of NF-κB signaling gene set and inflammatory response gene set. (D) ES2 and SKOV3 cells were treated with either vehicle or Liq or S-equol for 24 h, and the selective genes representing each pathway were validated by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Journal: Oncotarget

    Article Title: Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer

    doi: 10.18632/oncotarget.18442

    Figure Lengend Snippet: Total RNA was isolated from the ES2 cells that were treated with either vehicle or Liq (100 μM) for 24 h and subjected to RNA sequencing. (A) Heat map of differentially expressed genes between vehicle and Liq is shown. (B) Differentially expressed genes were subjected to pathway analysis using IPA software, and the selected top canonical pathways are shown. Analysis of molecular and cellular functions of differentially expressed genes are shown. (C) Gene set enrichment analysis (GSEA) testing correlation of Liq-regulated genes with signatures of NF-κB signaling gene set and inflammatory response gene set. (D) ES2 and SKOV3 cells were treated with either vehicle or Liq or S-equol for 24 h, and the selective genes representing each pathway were validated by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Article Snippet: Human OCa cell lines ES2, SKOV3, and BG-1 were obtained from the American Type Culture Collection (ATCC) and were maintained as per ATCC guidelines.

    Techniques: Isolation, RNA Sequencing, Software, Quantitative RT-PCR

    (A) ES2, SKOV3, and SKOV3-TR cells were transfected with NF-κB-luc reporter plasmid and grown for 24 h. Then, cells were treated with ERβ agonists and reporter activity was measured after 24 h. ES2 (B) and SKOV3 (C) cells were treated with Liq or S-equol (100 μM) for 24 h, and the expression status of NF-κB target genes was analyzed by using RT-qPCR. (D) ES2 cell lysates were used in pull-down assays using GST or ERβ-GST, and interaction of ERβ with p65 was analyzed by Western blotting. (E) ES2-ERβ-FLAG cell lysates were subjected to immunoprecipitation with IgG or FLAG antibodies and the interaction of ERβ with p65 was confirmed by Western blotting. (F) SKOV3 cells were transduced with empty vector or ERβ-FLAG expression vector and the expression of NF-κB target genes was analyzed by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Journal: Oncotarget

    Article Title: Therapeutic utility of natural estrogen receptor beta agonists on ovarian cancer

    doi: 10.18632/oncotarget.18442

    Figure Lengend Snippet: (A) ES2, SKOV3, and SKOV3-TR cells were transfected with NF-κB-luc reporter plasmid and grown for 24 h. Then, cells were treated with ERβ agonists and reporter activity was measured after 24 h. ES2 (B) and SKOV3 (C) cells were treated with Liq or S-equol (100 μM) for 24 h, and the expression status of NF-κB target genes was analyzed by using RT-qPCR. (D) ES2 cell lysates were used in pull-down assays using GST or ERβ-GST, and interaction of ERβ with p65 was analyzed by Western blotting. (E) ES2-ERβ-FLAG cell lysates were subjected to immunoprecipitation with IgG or FLAG antibodies and the interaction of ERβ with p65 was confirmed by Western blotting. (F) SKOV3 cells were transduced with empty vector or ERβ-FLAG expression vector and the expression of NF-κB target genes was analyzed by using RT-qPCR. Data are represented as mean ± SE. * p<0.05; ** p<0.01; *** p<0.001.

    Article Snippet: Human OCa cell lines ES2, SKOV3, and BG-1 were obtained from the American Type Culture Collection (ATCC) and were maintained as per ATCC guidelines.

    Techniques: Transfection, Plasmid Preparation, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunoprecipitation, Transduction